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Bullett Raja  Hindi HD - Region C.Short Tandem Repeat analysis for paternity exclusion testing in comparative studies on wild female fitness in invertebrates.
The main goals of this paper are to present a way to use modern short tandem repeat (STR) analysis to facilitate studies of inbreeding avoidance in wild invertebrates and to report a preliminary effort to test an important concept in the field of behavioral evolution: that mating can have costs to female fitness. In this effort, females from a population of Daphnia pulex were collected during different seasons of the year and their reproductive fitness was tested in a competition experiment where each female was paired with a "dummy" male (no egg production) or with one of several "real" males (n=6). We used the 13 STRs that we developed and tested in this species, in addition to eight microsatellites and two mitochondrial loci. For each female, we examined the number of alleles in the offspring of this pairing, and used STRs that we expected to be informative in differentiating between alleles in the individuals. We also used STRs that were expected to be uninformative because the alleles were expected to be similar between the individuals (null STRs). In an analysis of this type of data, a set of the unique STR markers can be used as a data reduction strategy to reveal patterns of inbreeding. The STRs that were informative for the paternity analysis were distributed throughout the genome. This method of STR analysis appears to be sensitive to the mechanisms of inbreeding avoidance and to the allele frequencies of STR markers in the population. These preliminary results suggest that the cost of mating can be an important mechanism of inbreeding avoidance in the field.Q:
Ubuntu boot issues after VirtualBox VMs upgrade
I've been using VirtualBox 4.2.6 for about a year now. There were a couple of updates a few weeks ago which allowed my VMs to boot up on the latest Ubuntu 13.10 (saucy) release.
Today, while doing a new install of Ubuntu 14.04 (trusty) on a VM, the VM won't boot. The VM is still running fine under VirtualBox ( 0b46394aab